RNAi is a specific, potent, and highly successful approach for loss-of-function studies in virtually all eukaryotic organisms. At Thermo Fisher Scientific, we have developed two types of small RNA molecules that function in RNAi: short interfering RNA (siRNA) molecules and microRNAs (miRNA). Thermo Fisher Scientific offers Invitrogen? products for RNAi analysis in vitro and in vivo, including libraries for high-throughput applications. Your choice of tool depends on your model system, the length of time you require knockdown, and other experimental parameters.
Featured RNAi categories
Effectively knock down gene expression using our proprietary Silencer Select, Stealth RNAi, and Silencer siRNAs
Analyze miRNA function with Invitrogen mirVana miRNA mimics and inhibitors
Find a complete solution for successful in vivo RNAi experiments including the highest nuclease stability Invitrogen? Ambion? In Vivo siRNA
Determine the best approach for delivering RNAi to your cells using either lipid-mediated transfection (Invitrogen Lipofectamine reagents) or the Invitrogen Neon Transfection System
Increase discovery with the widest selection of predefined and custom RNAi libraries available
Find a complete solution for successful in vivo RNAi experiments including the highest nuclease stability Ambion In Vivo siRNA
Reagents & kits to achieve transient or stable target knockdown in hard-to-transfect cells including Invitrogen BLOCK-iT system products
This Nature publication from NCATS, NIH demonstrates the utility of Silencer Select Genome-Wide siRNA library for arrayed high throughput screening in effectively identifying novel drug targets for Parkinson’s disease. The workflow adopted for this effort also was dependent on other products such as Lipofectamine RNAiMAX and Gibco Media for its success. Final validation of lead drug targets was accomplished by creating knockout cell lines using the TAL-effector endonuclease (TALEN) technology.